Direct Immunofluorescence in Laboratory Diagnosis

What technique is used in laboratories to diagnose diseases related to kidney, skin, and other organ systems?

A. direct immunofluorescence.

B. indirect immunofluorescence.

C. fluorescence polarization.

D. autofluorescence.

Answer:

The correct answer will be option-A

Direct immunofluorescence or the primary fluorescence is a technique used in the laboratories to diagnose the disease related to kidney, skin, and other organ systems.

The technique is performed by applying antibody–fluorophore conjugate molecules to the antigen expressed by the cells of the infected tissue.

When the tissue is observed with a fluorescent microscope, the fluorophore emits light of its own wavelength which helps analyze the antigen and the staining pattern in the tissue.

Thus, option-A is the correct answer.